Issue |
J Extra Corpor Technol
Volume 20, 1988
Proceedings of AmSECT’s 26th International Conference
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Page(s) | 94 - 98 | |
DOI | https://doi.org/10.1051/ject/198820S094 | |
Published online | 25 August 2023 |
Original Article
Low Volume Isolated IN SITU Lung Perfusion: Results of Studies of Metabolic Stability
The Milton S. Hershey Medical Center, The Pennsylvania State University, Hershey, Pennsylvania
* Direct communications to: Annette Basile-Borgia, Cardiopulmonary Perfusion Resources, Ltd., 41 Gettysburg Pike, Mechanicsburg, P A 17055.
Non-ventilatory lung functions involve uptake and release of compounds across the pulmonary vascular bed. Alterations in these functions may be readily assessed in an isolated, ventilated, small mammal lung perfusion preparation. A previously documented preparation is known to maintain metabolic stability of in situx rat lungs for at least 240 minutes. That model, developed by Watkins and Rannels, requires a rotating drum oxygenator which necessitates the use of 100 ml of perfusate buffer. The effects of 100 ml dilution limit the ability to measure uptake and release of metabolites because increased sensitivity of assay procedures or more prolonged experiments are required. A recently developed preparation requires only 25 ml of recirculating perfusate, clearly reducing substrate and metabolite dilution, and enhancing the sensitivity for detection of changes in pulmonary metabolism.
The intent of this study was to test the vascular permeability and metabolic stability of this low volume lung perfusion model. The low volume, in situ lung perfusion model remained stable for as long as 3 hours. Metabolic stability of the model was demonstrated by measurements of water balance, glucose uptake, lactate production, and protein synthesis. The clinical relevance of isolated lung perfusion is discussed with specific reference to metabolic functions of the lung.
© 1988 AMSECT
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