Issue |
J Extra Corpor Technol
Volume 21, Number 2, June 1989
|
|
---|---|---|
Page(s) | 52 - 55 | |
DOI | https://doi.org/10.1051/ject/1989212052 | |
Published online | 23 August 2023 |
Original Article
Endothelial Cell Stress with Arterial Re-entry During Bypass
The Mechanisms of Perfusion IV Meeting, Tampa, Florida
* Address correspondence to: Philip D. Beckley, 1583 Perry Street, Columbus, 011 43210. Supported in part by a grant from Sarns Incorporated/3M
High velocity arterial blood re-entry during cardiopulmonary bypass is known to be associated with numerous potential deleterious effects. One of these may be endothelial cell stress with resulting intimal injury. Previous studies by others would suggest that endothelial cell injury of this type could result in long-term vascular disease. To test the hypothesis that arterial reentry during cardiopulmonary bypass will induce endothelial injury, six mongrel dogs were divided into three study pairs: control (no bypass), continuous (non-pulsatile) bypass, and pulsatile bypass. Evans blue dye was used as a marker of aortic endothelial cell injury. Following each experimental period, the aorta was removed en bloc and examined for Evans Blue Dye staining. Sections of aortic tissue were also examined histologically for cellular damage.
All subjects tolerated the study period without incident. Gross tissue examination of the control subject aortic segments revealed no Evans Blue Dye staining. Histologic examination of these segments was unremarkable. Gross tissue examination of the bypass subject aortic segments revealed Dye staining in all cases with the pulsatile bypass pair staining more extensively and heavily than the continuous bypass pair. Histologic examination of the stained tissue showed acute and focal endothelial denudation (J. Extra-Corporeal Technol. 21(2): 52-55, 1989).
Key words: Aortic endothelial injury / blood velocity / pulsatile pump / Evans Blue Dye
© 1989 AMSECT
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.