Issue |
J Extra Corpor Technol
Volume 22, 1990
Proceedings of AmSECT’s 28th International Conference
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Page(s) | 57 - 61 | |
DOI | https://doi.org/10.1051/ject/199022S057 | |
Published online | 23 August 2023 |
Original Article
Effect of Serum Precoat on Complement Activation in Membrane Oxygenators: An In Vitro Study
Perfusion Techology Program, College of Pharmacy and Allied Health Professions, Northeastern University, Boston, MA
* Address correspondence to: Patrick F. Plunkett, Ed.D., 234 Mugar Life Science Building, Northeastern University, Boston, MA 02115
Previous studies have shown that extracorporeal circulation devices can activate the complement cascade via the alternative pathway. Anaphylatoxins (C3a and C5a) generated by complement activation may lead to pulmonary leukocyte sequestration, pulmonary edema, granulocytopenia and microvascular lung damage. This is of particular concern in patients on cardiopulmonary bypass. The purpose of this study was to examine the impact on complement activation of precoating membrane oxygenators with serum. A pool of recalcified human serum was prepared from fresh frozen plasma stored in citrate phosphate dextrose. SciMed membrane oxygenators (N=4) were primed with 200 ml aliquots of pooled serum which were recirculated through the oxygenators for 180 minutes at 37° C. The primary recirculation serum C3a levels (mean ± SD) measured by radioimmunoassay techniques at 0, 60, 120, and 180 minutes were 122.25 ± 26.22, 430.25 ± 164.39, 456.0 ± 154.16 and 577.5 ± 163.07 ng/ml respectively. Each oxygenator was then flushed with saline and reprimed with a fresh 200 ml aliquot of pooled serum which was recirculated for 180 minutes at 37° C. The secondary recirculation serum C3a levels (mean ± SD) measured at 0, 60, 120, and 180 minutes were 130.75 ± 15.67, 213.25 ± 58.24, 283.75 ± 27.24 and 301.75 ± 19.18 ng/ml respectively. Serum C3a levels were higher in the primary serum than the secondary serum in all oxygenators. These results suggest that preconditioning the oxygenator may be a way to moderate complement activation during cardiopulmonary bypass.
Key words: Complement / membrane oxygenator
© 1990 AMSECT
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