Effect of Potassium-Channel Openers on the Release of Endothelium-Derived Hyperpolarizing Factor in Porcine Coronary Arteries Stored in Cold Hyperkalemic Solution

Anesthesiology and Cardiopulmonary Bypass Laboratory, Cardiovascular Institute and Fuwai Hospital, CAMS and PUMC, Beijing, People’s Republic of China

^* Address correspondence to: Cun Long, MD, Anesthesiology and Cardiopulmonary Bypass Laboratory, Cardiovascular Institute and Fuwai Hospital, CAMS and PUMC, Beijing 100037 People’s Republic of China. E-mail: fuwaipb@mx.cei.gov.cn

Received: 25 March 2001
Accepted: 18 December 2001

Abstract

Hyperkalemic solution is widely used to protect the myocardium during open-heart surgery or to preserve donor hearts during heart or heart/lung transplants. The inhibitory effects of hyperkalemic solution on the release of endotheliumderived hyperpolarizing factor (EDHF) of coronary arteries following deep hypothermic storage (4°C) has been well studied. However, it has not been established whether potassium channel openers have protective effects on the coronary endothelial function after cold storage. This study was designed to examine this. Porcine coronary artery rings were studied in organ baths. Relaxation in response to the EDHF stimulus A23187 (nonreceptor-mediated stimulus calcium ionophore) in thromboxane A2 mimetic U46619 (30 nmol/L)-induced precontraction after incubation with hyperkalemic solution (20 mmol/L) with nicorandil (10 μmol/L) (either at 37°C in the oxygenated organ chamber or at 4°C in a refrigerator for 6 h) was compared with the control. There was significant difference between hyperkalemia group and hyperkalemia with nicorandil group under normothermia (p = .04). The difference was significant in the same solution between normothermia and hypothermia. After incubation in hyperkalemic solution without or with nicorandil, the A23187induced relaxation was 32.8% ± 9.1% and 72.6% ± 16.9%, respectively (N = 8, p < .01). Potassium channel opener can attenuate the inhibitory effect of hyperkalemic solution on the release of EDHF after cold storage.