Issue |
J Extra Corpor Technol
Volume 19, Number 3, September 1987
|
|
---|---|---|
Page(s) | 245 - 257 | |
DOI | https://doi.org/10.1051/ject/1987193245 | |
Published online | 29 August 2023 |
Original Article
Age Related Differences in Oxygen Free Radical Injury during Myocardial Ischemia
The Department of Surgery Thoracic Division, SUNY Health Science Center Syracuse, New York
* Direct communications to: Alfred H. Stammers, Department of Surgery, SUNY Health Science Center, Syracuse, NY 13210
(J. Extra-Corpor. Technol. 19[3] p. 245-257 Fall 1987, 42 ref.) Myocardial protection with an oxygenated cardioplegic solution was studied in mature and immature animals. Isolated adult and neonatal rabbit hearts were exposed to 90 minutes of hypothermic 30°C ischemia, and one of the following treatments: multidose oxygenated (pO2<650 torr) cardioplegia, multidose nonoxygenated (pO2<200 torr) cardioplegia, and a noncardioplegic Krebs-Henseleit solution. Upon reperfusion, adult hearts that had not received either cardioplegia treatment failed to recover postischemic hemodynamic function. However, neonates given the same treatment were able to recover 74.0 ± 2.4% (Mean ± SEM) of preischemic cardiac output and 69.2 ± 5.7% recovery of stroke work. Oxygenated cardioplegia administered to adult and neonatal hearts resulted in postischemic cardiac output recovery of 90.8 ± 2.4% and 86.5 ± 8.6%, respectively. Cardiac output recovery in nonoxygenated cardioplegia groups was 105.3 ± 3.9% in the adults, and 95.2 ± 6.6% in the neonates. Coronary sinus creatine kinase was substantially elevated in all adult groups, but not in the cardioplegia treated neonates. Membrane lipid peroxidation was assessed by measuring malondialdehyde in myocardial tissue homogenates. Malondialdehyde remained at control levels across all neonatal groups, but in the mature hearts the nonoxygenated cardioplegia group had significantly lower peroxidative products than the oxygenated hearts. The results of this study indicate an increased susceptibility of the mature myocardium to both the damaging effects of ischemia and free oxygen radicals. Administration of an oxygenated cardioplegic solution failed to provide adequate protection during moderate hypothermic arrest, and resulted in increased membrane peroxidative activity.
Key words: cardioplegia / oxygenated / infant / myocardial preservation
© 1987 AMSECT
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