Issue |
J Extra Corpor Technol
Volume 37, Number 2, June 2005
|
|
---|---|---|
Page(s) | 196 - 200 | |
DOI | https://doi.org/10.1051/ject/200537196 | |
Published online | 15 June 2005 |
Scientific Article
Quality of Thrombin Produced From the Patient’s Own Plasma Using the TPD™, a New Thrombin-Processing Device
* Thermogenesis Corp., Rancho Cordova, California
† Department of Laboratory Medicine and Pathobiology, St. Michael’s Hospital
‡ Departments of Pharmacology, Medicine and Laboratory Medicine and Pathobiology, University of Toronto, Canadian Blood Services
§ Toronto Platelet Immunobiology Group, Toronto, Ontario, Canada
Address correspondence to: Dr. John W. Semple, St. Michael’s Hospital, 30 Bond Street, Toronto, ON, Canada, M5B 1W8. E-mail: semplej@smh.toronto.on.ca
Thrombin derived from bovine sources commonly is used to arrest bleeding during surgical procedures. However, complications such as postoperative hemorrhage can occur because of the development of cross-reactive anti-bovine antibodies that inhibit human coagulation factor V. It would thus be advantageous to develop techniques to generate human thrombin. This study evaluated thrombin produced from human plasma using a new Thrombin-Processing Device (TPD™). Plasma was introduced into the TPD, mixed with an ethanol/CaCl2 reagent, incubated for 1 h, and the harvested thrombin was assayed for activity and the ability to activate platelets by in vitro assays. TPD-produced thrombin activity was found to be 51.8 ± 12.4 IU/mL (n = 145). TPD-produced thrombin also stimulated P-selectin (CD62) expression (83 ± 13% of the platelet population) and Annexin V binding (10.3 ± 2% of the platelet population) on platelets in a similar fashion to commercial thrombin (P-selectin expression: 88 ± 3%; Annexin-V binding: 11.4 ± 3%). Compared with CaCl2 and batroxobin, TPDproduced thrombin had a significantly greater ability to activate platelets. TPD-produced thrombin from human plasma has consistent activity and significantly activates platelets and, thus, may have attractive applications such as the production of autologous thrombin for surgical patients.
Key words: human thrombin / platelet activation / autologous
© 2005 AMSECT
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